Cell:哺乳动物microRNA表达图谱- 生物研究- 生物谷
来源:百度文库 编辑:神马文学网 时间:2024/04/29 16:41:19
该图谱对来自人类和啮齿类的26种正常、肿瘤组织的细胞的microRNA表达进行了定量测定。“他们掌握了一种极为有用的资源,”Dartmouth医学院Victor Ambros说(未参与实验),他们在研究中收集的组织种类非常多,并对这些组织中的microRNA的特征进行了系统性分析。 新绘制的microRNA表达图谱含有许多关于microRNA结构、序列、表达方式和进化的有用信息。Ambros认为这是一个基本数据库,为研究发育和疾病有关的microRNA世界提供了重要线索。
microRNA是动物、植物和病毒中普遍存在的非编码小RNA,通过干扰mRNA的功能调节基因表达。科研人员已经鉴定出多种哺乳动物microRNA,但对这些microRNA中大多数的表达水平和特征都是未知的。
洛克菲勒大学Pablo Landgraf率领的研究小组克隆并测序了256个哺乳动物小RNA数据库中的30多万个序列,找到了大约400个在任何组织都表达的microRNA——比之前预期的要少。约翰霍普金斯大学Joshua Mendell说,这个结果提示在其它研究中找到的几个百microRNA是未知的。
Landgraf等还发现他们测序的microRNA克隆中,97%的来自不到300个microRNA。文章共同作者、瑞士Basel大学Mihaela Zavolan认为许多microRNA在各种组织间是低背景水平表达的(low background level),现在不能确定这些“痕量”microRNA的功能,但很有可能大多数microRNA的功能是不相关的。他们还发现这些稀少的microRNA在物种间的保守性很差。
研究人员发现组织或者细胞特异的microRNA种类很少,相反,细胞系和组织之间的差异经常局限在一小部分普遍存在的microRNA的表达水平的变化。Mendell说缺乏细胞、组织特异的microRNA并不意味着microRNA没有区分肿瘤起源、组织或细胞的作用。真正重要的是一个给定样本表达的所有microRNA。
此次研究只鉴别出33种新的哺乳动物microRNA。“这真是一件重量级研究,”Ambros说,意味着我们渐渐补全了哺乳动物microRNA的目录,至少是丰富了主要器官和组织的microRNA,还有可能某些功能重要的microRNA只在个别种类细胞中表达,所以没有被检测到。
Table 1 . miRNAs Identified in This Study and Not Listed in miRBase (Release 9.1)
Table 1. miRNAs Identified in This Study and Not Listed in miRBase (Release 9.1)
Mouse miRNA
miR-872__FM AAGGUUACUUGUUAGUUCAGG 11
miR-872 AAGGUUACUUGUUAGUUCAGG 394
miR-872 AAGGUUACUUGUUAGUUCAGG 373
miR-879 AGAGGCUUAUAGCUCUAAGCC 33
miR-879 AGAGGCUUAUAGCUCUAAGCC 5
miR-8822 AGGAGAGAGUUAGCGCAUUAGU 1
miR-882a6 AUGGGAAUGUUAGAGCAUUAGC 0
miR-882b AUGGCAAUGUUAGAGCAUUAGC 0
miR-874 CUGCCCUGGCCCGAGGGACCGA 17
miR-874 CUGCCCUGGCCCGAGGGACCGA 2
miR-874 CUGCCCUGGCCCGAGGGACCGA 0
miR-877 GUAGAGGAGAUGGCGCAGGG 40
miR-877 GUAGAGGAGAUGGCGCAGGG 6
miR-877 GUAGAGGAGAUGGCGCAGGG 2
miR-297c3 AUGUAUGUGUGCAUGUACAUGU 16
| miR-743b4 GAAAGACAUCAUGCUGAAUAGA 8 | miR-743b7 GAAAGACACCAUACUGAAUAGA 0
| miR-883a UAACUGCAACAGCUCUCAGUAU 6
| miR-883b UAACUGCAACAUCUCUCAGUAU 1 | miR-883 UAACUGCAACAUCUCUCAGUAU 0
| miR-880 UACUCCAUCCUCUCUGAGUAGA 119 | miR-880 UACUCCAUUCAUUCUGAGUAGA 3
| miR-878 GCAUGACACCACACUGGGUAGA 22 | miR-878 GCAUGACACCAUACUGGGUAGA 1
| miR-874 AACUGUGUCUUUUCUGAAUAGA 60 | miR-881 AACUGUGGCAUUUCUGAAUAGA 0
miR-871__FM UAUUCAGAUUAGUGCCAGUCAUG 2 | miR-871 UAUUCAGAUUAGUGCCAGUCAUG 11 | miR-871 UAUUCAGAUUGGUGCCGGUCACA 0 | miR-8901 UACUUGGAAAGGCAUCAGUUG 10
| miR-888 UACUCAAAAAGCUGUCAGUCA 16
| miR-465d UAUUUAGAAUGGUACUGAUGUG 0
| miR-892b CACUGGCUCCUUUCUGGGUAGA 14 | miR-465c UAUUUAGAAUGGCGCUGAUCUG 42
| miR-891b UGCAACUUACCUGAGUCAUUGA 4
| miR-891a UGCAACGAACCUGAGCCACUGA 24
| miR-466f5 UACGUGUGUGUGCAUGUGCAUG 7
| miR-466e GAUGUGUGUGUACAUGUACAUA 1.5
| miR-466b GAUGUGUGUGUACAUGUACAUG 7
| miR-466b8 UAUGUGUGUGUGUAUGUCCAUG 1
| miR-466c GAUGUGUGUGUGCAUGUACAUA 11
| miR-466c UGUGAUGUGUGCAUGUACAUG 2
| miR-467e AUAAGUGUGAGCAUGUAUAUGU 125
| miR-466d UGUGUGUGCGUACAUGUACAUG 4
| miR-466g AUACAGACACAUGCACACACA 2
| miR-466h UGUGUGCAUGUGCUUGUGUGUA 4
miR-885 UCCAUUACACUACCCUGCCUCU 5
miR-887 GUGAACGGGCGCCAUCCCGAGG 3
miR-886 CGCGGGUGCUUACUGACCCUU 16
The predominantly cloned sequence and orthologs are indicated. Bars beside the name indicate clustered miRNAs.
[1] Part of the cluster-hsa-mir-888(6), epididymis-specific expression.
[2] Part of the cluster-mmu-mir-134(33), enriched in embryonic tissue.
[3] Part of the cluster-mmu-mir-297a-1(46).
[4] Part of the cluster-mmu-mir-463(19), epididymis (testis) enriched expression.
[5] Part of the cluster-mmu-mir-297a-3(43).
[6] Part of the cluster-rno-mir-134(41).
[7] Part of the cluster-rno-mir-463(12).
[8] Part of the cluster-rno-mir-297c(4); Abbreviations are as follows: FM: “from mouse”; the same miRNA sequence as in mouse was cloned but could not be found in the current human assembly.
原始出处:
Cell , Vol 129, 1401-1414, 29 June 2007