神马文学网蝴蝶兰病毒的PCR 引物
来源：百度文库 编辑：神马文学网 时间：2024/05/06 10:30:35
编号
病毒名称
引物序列F/R
1
INSV (Impatiens necrotic spot virus)
凤仙花坏死斑病毒
INSV-F1 ATTTAACACAACACAAAGCAAAC 23nt
INSV-R1 CGAATTTCACAAGTTTAATGATTT 24
INSV-F2 CAGTGCATCAAAATTATATCTAGCCG 26
INSV-R2 TACACTAGTTCAAATGTTCTTTCTAC 26
2
ORSV(Odontoglossum ringspot virus) 齿兰环斑病毒
ORSV-F1 ACGCACAATCTGATCCGTA 19
ORSV-R1 ATCCGCAGTGAAAACCC 17
3
CYMV (Cymbidium mosaic virus)
建兰花叶病毒
CYMV –F1 GAAATAATCATGGGAGAGCC 20
CYMV –R1 AGTTTGGCGTTATTCAGTAGG 21
4
ORSV和
CYMV
ORSV-F2 CCACCGAACTTACTGAAG 18
ORSV-R2 TGTCGTAAGGTTGACCACTC 20
CyMV-F2 GCTACCGCCGCCATCGTCAT 20
CyMV-R2 GCGCAGCACGTTCACGGTCA 20
5
TSWV
(Tomato spotted wilt virus）番茄斑萎病毒
TSWV-F1 CCCGGA TCCA GA GCAA T 17
TSWV-R1 CA C TGGA TCC TTTTG TTTTTG TTTTTTG 28
6
OFV
(Orchid fleck virus)
兰花斑点病毒
OFV-F1 TACTGATGCTGATGCCACTCTTT 23
OFV-R1 ACCCAACTGGGAGAGACTCTATT 23
7
CalMMV (Calanthe mild mosaic virus) 虾脊兰轻型花叶病毒
CalMMV-F1 GGA TGC TGG ACT CAG TGC T 19
CalMMV-R1 GTGCATATTCGTGTTCACAT 20
CalMMV-F2 GCT GGA CTC AGT GCT GAG A 19
CalMMV-R2 GTTCTCCTCCTGCGTTGCTAT 21
8
CMV (Cucumber mosaic virus) 黄瓜花叶病毒
CMV –F1 CGG ATG CTA ACT TTA GAG TCT TGT 24
CMV –R1 GAATGCGTTGGTGCTCGAT 19
9
DVNV (Dendrobium vein necrosis virus) 石斛脉坏死病毒
无
10
PhCSV (Phalaenopsis chlorotic spot virus) 蝴蝶兰褪绿斑病毒
PhCSV –F1 CAA GGG CAA GAA GGC AGT AT 20
PhCSV –R 1 CATACGAGCATTGACGTCAGA 21
PhCSV –R 2 GTTCCCATCCAAACCAAATAGT 22
11
Potexvirus属通用引物
potex-F1 CAYCARCARGCMAARGAYGA 20
potex-R1 AGCATRGCNSCRTCYTG 17
12
Tospoviruses番茄斑萎属简并引物
Tospo-F1 CC(A⁄T) TT(A⁄G) ACA GT(A⁄T⁄G)GA(A⁄G) ACAT
Tospo-R1 CAT(A⁄T⁄G)GC (A⁄G)CA AGA (A⁄G)TG (A⁄G)TA(A⁄T⁄G)AC AGA
Tospo-R2 TCATC(A⁄G) GA(A⁄G) TG(A⁄T⁄G⁄C) AC(A⁄C) ATCCA(T⁄C) CT
13
辣椒褪绿病毒（Capsicum chlorosis virus）CaCV
CaCV-F1 ATGTCTACTGCAAAGAGTGC 20
CaCV-R1 CACAGAAATCAACTAAACCT 20
CaCV-F2 TCACACTTCTATAGATGAGCT 21
CaCV-R2 GACTTTCACAAATTGCTTGA 20
14
香石竹斑驳病毒（Carnation mottle virus）CarMV
CarMV-F1 AATCCCACTGTGCAAACATT 20
CarMV-R1 TAACCGAAGGGACCACATAC 20
15
Potyvirus简并引物
Poty-F1 GTTTTCCCAGTCACGAC 17
Poty-R1 GGXAAYAAYAGYGGXCAZCC 20
16
TMV烟草花叶病毒
Tobacco mosaic virus
TMV-F1 ATGTCTTACAGTATCACTACTCC 23
TMV-R1 TATAAGATCCGGTTCCTCTG 20
17
向日葵褪绿斑驳病毒（sunflower chlorotic mottle virus）
SuCM-F1 GAGGCGTGGGGCTATCC
SuCM-R1 AAAAGTAGTACAGGAAAAGCC
18
Potyvirus简并引物
Poty-F2 ATGGTHTGGTGYATHGARAAYGG
Poty-R2 TGCTGCKGCYTTCATYTG
19
TSWV
(Tomato spotted wilt virus）番茄斑萎病毒
TSWV-F2
TSWV-R2 CA C TGGA TCC TTTTG TTTTTG TTTTTTG 28
病毒名称
引物序列F/R
1
INSV (Impatiens necrotic spot virus)
凤仙花坏死斑病毒
INSV-F1 ATTTAACACAACACAAAGCAAAC 23nt
INSV-R1 CGAATTTCACAAGTTTAATGATTT 24
INSV-F2 CAGTGCATCAAAATTATATCTAGCCG 26
INSV-R2 TACACTAGTTCAAATGTTCTTTCTAC 26
2
ORSV(Odontoglossum ringspot virus) 齿兰环斑病毒
ORSV-F1 ACGCACAATCTGATCCGTA 19
ORSV-R1 ATCCGCAGTGAAAACCC 17
3
CYMV (Cymbidium mosaic virus)
建兰花叶病毒
CYMV –F1 GAAATAATCATGGGAGAGCC 20
CYMV –R1 AGTTTGGCGTTATTCAGTAGG 21
4
ORSV和
CYMV
ORSV-F2 CCACCGAACTTACTGAAG 18
ORSV-R2 TGTCGTAAGGTTGACCACTC 20
CyMV-F2 GCTACCGCCGCCATCGTCAT 20
CyMV-R2 GCGCAGCACGTTCACGGTCA 20
5
TSWV
(Tomato spotted wilt virus）番茄斑萎病毒
TSWV-F1 CCCGGA TCCA GA GCAA T 17
TSWV-R1 CA C TGGA TCC TTTTG TTTTTG TTTTTTG 28
6
OFV
(Orchid fleck virus)
兰花斑点病毒
OFV-F1 TACTGATGCTGATGCCACTCTTT 23
OFV-R1 ACCCAACTGGGAGAGACTCTATT 23
7
CalMMV (Calanthe mild mosaic virus) 虾脊兰轻型花叶病毒
CalMMV-F1 GGA TGC TGG ACT CAG TGC T 19
CalMMV-R1 GTGCATATTCGTGTTCACAT 20
CalMMV-F2 GCT GGA CTC AGT GCT GAG A 19
CalMMV-R2 GTTCTCCTCCTGCGTTGCTAT 21
8
CMV (Cucumber mosaic virus) 黄瓜花叶病毒
CMV –F1 CGG ATG CTA ACT TTA GAG TCT TGT 24
CMV –R1 GAATGCGTTGGTGCTCGAT 19
9
DVNV (Dendrobium vein necrosis virus) 石斛脉坏死病毒
无
10
PhCSV (Phalaenopsis chlorotic spot virus) 蝴蝶兰褪绿斑病毒
PhCSV –F1 CAA GGG CAA GAA GGC AGT AT 20
PhCSV –R 1 CATACGAGCATTGACGTCAGA 21
PhCSV –R 2 GTTCCCATCCAAACCAAATAGT 22
11
Potexvirus属通用引物
potex-F1 CAYCARCARGCMAARGAYGA 20
potex-R1 AGCATRGCNSCRTCYTG 17
12
Tospoviruses番茄斑萎属简并引物
Tospo-F1 CC(A⁄T) TT(A⁄G) ACA GT(A⁄T⁄G)GA(A⁄G) ACAT
Tospo-R1 CAT(A⁄T⁄G)GC (A⁄G)CA AGA (A⁄G)TG (A⁄G)TA(A⁄T⁄G)AC AGA
Tospo-R2 TCATC(A⁄G) GA(A⁄G) TG(A⁄T⁄G⁄C) AC(A⁄C) ATCCA(T⁄C) CT
13
辣椒褪绿病毒（Capsicum chlorosis virus）CaCV
CaCV-F1 ATGTCTACTGCAAAGAGTGC 20
CaCV-R1 CACAGAAATCAACTAAACCT 20
CaCV-F2 TCACACTTCTATAGATGAGCT 21
CaCV-R2 GACTTTCACAAATTGCTTGA 20
14
香石竹斑驳病毒（Carnation mottle virus）CarMV
CarMV-F1 AATCCCACTGTGCAAACATT 20
CarMV-R1 TAACCGAAGGGACCACATAC 20
15
Potyvirus简并引物
Poty-F1 GTTTTCCCAGTCACGAC 17
Poty-R1 GGXAAYAAYAGYGGXCAZCC 20
16
TMV烟草花叶病毒
Tobacco mosaic virus
TMV-F1 ATGTCTTACAGTATCACTACTCC 23
TMV-R1 TATAAGATCCGGTTCCTCTG 20
17
向日葵褪绿斑驳病毒（sunflower chlorotic mottle virus）
SuCM-F1 GAGGCGTGGGGCTATCC
SuCM-R1 AAAAGTAGTACAGGAAAAGCC
18
Potyvirus简并引物
Poty-F2 ATGGTHTGGTGYATHGARAAYGG
Poty-R2 TGCTGCKGCYTTCATYTG
19
TSWV
(Tomato spotted wilt virus）番茄斑萎病毒
TSWV-F2
TSWV-R2 CA C TGGA TCC TTTTG TTTTTG TTTTTTG 28